Journal: Molecules

Article Title: Protective Effect of Phenolic Compounds Isolated from Mugwort ( Artemisia argyi ) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1

doi: 10.3390/molecules24010195

Figure Lengend Snippet: Effects of methyl caffeate isolated from Artemisia argyi , NAC and artemetin on apoptosis in LLC-PK1 cells exposed to 25 mg/mL of iodixanol scored by an image-based cytometric assay, Hoechst 33342 staining and DCFH-DA staining. ( A ) Representative images of apoptosis detection and intracellular reactive oxygen species (ROS) levels. ( B ) Percentage of Annexin V stained apoptotic cells. ( C ) Fluorescence intensity of 2′, 7′- dichlorodihydrofluorescein (DCF). Control cells were treated with the vehicle only (mean ± SD, n = 3, * p < 0.05 compared to the iodixanol-treated group).

Article Snippet: Tali image-based cytometric assay kit was purchased from Invitrogen (Temecula, CA, USA).

Techniques: Isolation, Staining, Fluorescence

Journal: World Journal of Gastrointestinal Oncology

Article Title: Antitumor activity of miR-188-3p in gastric cancer is achieved by targeting CBL expression and inactivating the AKT/mTOR signaling

doi: 10.4251/wjgo.v15.i8.1384

Figure Lengend Snippet: miR-188-3p induction of apoptosis and autophagy of gastric cancer cells. A and B: Flow cytometric/Annexin V-PE/7-AAD assay. AGS and BGC-823 cells were seeded in six-well plates for transfection with miR-188-3p mimics, inhibitor, or their NC and assayed for tumor cell apoptosis. b P < 0.01; C: qRT-PCR. AGS and BGC-823 cells were plated for transfection with miR-188-3p mimics, inhibitor, or their NC and the qRT-PCR assay was performed. b P < 0.01 and c P < 0.001; D and E: Western blot. AGS and BGC-823 cells were plated in 10 cm dishes for transfection with miR-188-3p mimics, inhibitor, or their NC and Western blot was conducted for the detection of Beclin-1 and LC3-II/LC3-I proteins.

Article Snippet: A flow cytometric Annexin V-PE apoptosis assay kit from BD Biosciences (Franklin Lakes, NJ, United States) was obtained to assess the level of apoptosis and cell cycle distribution of gastric cancer cells after gene transfection.

Techniques: Transfection, Quantitative RT-PCR, Western Blot

Journal: World Journal of Gastrointestinal Oncology

Article Title: Antitumor activity of miR-188-3p in gastric cancer is achieved by targeting CBL expression and inactivating the AKT/mTOR signaling

doi: 10.4251/wjgo.v15.i8.1384

Figure Lengend Snippet: CBL antagonization of miR-188-3p reduced gastric cancer cell proliferation and induced gastric cancer cell apoptosis and autophagy in vitro . A and B: Flow cytometry. AGS and BGC-823 cells were transfected with miR-188-3p mimics, miR-188-3p/CBL cDNA, or m-NC, miR-188-3p mimics + vector-only, after which the flow cytometric apoptosis assay was performed. c P < 0.001; C: qRT-PCR. AGS and BGC-823 cells were transfected with miR-188-3p mimics, miR-188-3p/CBL cDNA, or m-NC, miR-188-3p mimics + vector-only, after which qRT-PCR analysis was conducted. c P < 0.001; D: CCK-8 assay. AGS and BGC-823 cells were seeded for transfection with miR-188-3p mimics, miR-188-3p/CBL cDNA, or m-NC, miR-188-3p mimics + vector-only, after which the CCK-8 assay was performed.

Article Snippet: A flow cytometric Annexin V-PE apoptosis assay kit from BD Biosciences (Franklin Lakes, NJ, United States) was obtained to assess the level of apoptosis and cell cycle distribution of gastric cancer cells after gene transfection.

Techniques: In Vitro, Flow Cytometry, Transfection, Plasmid Preparation, Apoptosis Assay, Quantitative RT-PCR, CCK-8 Assay

Journal: Translational Neuroscience

Article Title: Protective Effect of Resveratrol Against Corticosterone-induced Neurotoxicity in PC12 Cells

doi: 10.1515/tnsci-2019-0038

Figure Lengend Snippet: Effects of RES on apoptosis rate in corticosterone-induced PC12 cells. The effect of RES on corticosterone-induced PC12 cell apoptosis based on flow cytometry analyses. The proportion (%) of cells in each quadrant is shown. Lower left quadrant (absence of both markers) indicates viable cells; upper left quadrant [propidium iodide (PI) positive] indicates cellular necrosis; upper right quadrant (Annexin V positive and PI positive) indicates late-stage apoptosis or cellular necrosis; lower right quadrant (Annexin V positive) indicates early-stage apoptosis. (B) Analysis of early-stage apoptosis, (n = 3). (C) Analysis of late-stage apoptosis, (n = 3). Data are expressed as a percentage of the control and the results are expressed as the means ± SD. * p < 0.05 and *** p < 0.01 vs model group.

Article Snippet: Resveratrol (Beijing Solarbio CO., Ltd, China); Corticosterone (Dalian Meilun Biotechnology CO., Ltd, China); Polyclonal rabbit antibodies (Bax, Bcl-2, caspase-3) (Santa Cruz Biotechnology CO., Ltd, CA); Cell Counting Kit-8 ( CCK-8) reagent (Colleague, Japan); Flow cell apoptosis assay kit (Nanjing Vazyme CO., Ltd, China).

Techniques: Flow Cytometry, Control

Journal: Anti-Cancer Drugs

Article Title: α-Asarone attenuates tumor-associated macrophages-induced gemcitabine resistance in pancreatic carcinoma via the transforming growth factor-beta 1/growth factor independent 1 axis

doi: 10.1097/CAD.0000000000001740

Figure Lengend Snippet: α-Asarone reverses TAM-CM-induced gemcitabine resistance in pancreatic cancer cells. (a) Relative cell viability of pancreatic cancer cells treated with different concentrations of gemcitabine, assessed by CCK-8 assay. (b) Effect of gemcitabine on the relative cell viability of pancreatic cancer cells in none (NC), TAM-CM, and TAM-CM + α-asarone treatment groups. (c) Apoptosis of pancreatic cancer cells analyzed by flow cytometry (Annexin V/PI). (d) Cell cycle distribution of pancreatic cancer cells analyzed by flow cytometry. CCK-8, Cell Counting Kit-8; CM, conditioned medium; CTGF, connective tissue growth factor; HMGB, high mobility group box; NC, negative control; PI, propidium iodide; TAM, tumor-associated macrophage.

Article Snippet: Cell Counting Kit-8 (CCK-8) kit, flow apoptosis assay kit, and cell cycle kit were purchased from Yeasen, Wuhan, China.

Techniques: CCK-8 Assay, Flow Cytometry, Cell Counting, Negative Control